| Antigen detectable with this ELISA kit: |
Basic (FGF2), Fibroblast Growth Factor 2 |
| Species reactivity: |
Bovine (Cattle), Bos taurus |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 6 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Basic (FGF2), Basic (FGF2), Basic (FGF2), Basic (FGF2) in the samples is then determined by comparing the O, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 2, The concentration of Fibroblast Growth Factor 2, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Fibroblast Growth Factor 2, D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Description: |
7 are often produced recombinant and shouldn&rsquo, Basic proteins pH>, t be precipitated by formic acid buffer |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
(FGF2), Fibroblast Growth Factor 2 |
| Short name: |
(FGF2) ELISA kit, Fibroblast Growth Factor 2 |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Basic (fibroblast growth factor 2 (basic)) Enzyme-linked immunosorbent assay reagent, Fibroblast Growth Factor 2 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BFGF and FGF-2 and FGFB and HBGF-2, BT, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046668 and regulation of retinal cell programmed cell death and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048598 and embryonic morphogenesis and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0048678 and response to axon injury and biological process this GO :0048864 and stem cell development and biological process this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0050918 and positive chemotaxis and biological process this GO :0051209 and release of sequestered calcium ion into cytosol and biological process this GO :0051726 and regulation of cell cycle and biological process this GO :0051781 and positive regulation of cell division and biological process this GO :0051897 and positive regulation of protein kinase B signaling and biological process this GO :0060045 and positive regulation of cardiac muscle cell proliferation and biological process this GO :0060128 and corticotropin hormone secreting cell differentiation and biological process this GO :0060129 and thyroid-stimulating hormone-secreting cell differentiation and biological process this GO :0060548 and negative regulation of cell death and biological process this GO :0060591 and chondroblast differentiation and biological process this GO :0060644 and mammary gland epithelial cell differentiation and biological process this GO :0060978 and angiogenesis involved in coronary vascular morphogenesis and biological process this GO :0061045 and negative regulation of wound healing and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0090263 and positive regulation of canonical Wnt signaling pathway and biological process, FGF2 and IDBG-37039 and ENSG00000138685 and 2247, Fgf2 and IDBG-140464 and ENSMUSG00000037225 and 14173, chemoattractant activity, nuclei, this GO :0000186 and activation of MAPKK activity and biological process this GO :0000187 and activation of MAPK activity and biological process this GO :0001525 and angiogenesis and biological process this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0001759 and organ induction and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0001938 and positive regulation of endothelial cell proliferation and biological process this GO :0002042 and cell migration involved in sprouting angiogenesis and biological process this GO :0005104 and fibroblast growth factor receptor binding and molecular function this GO :0005125 and cytokine activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0006661 and phosphatidylinositol biosynthetic process and biological process this GO :0006935 and chemotaxis and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007399 and nervous system development and biological process this GO :0007568 and aging and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008201 and heparin binding and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009792 and embryo development ending in birth or egg hatching and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010001 and glial cell differentiation and biological process this GO :0010595 and positive regulation of endothelial cell migration and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0010764 and negative regulation of fibroblast migration and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0021762 and substantia nigra development and biological process this GO :0021940 and positive regulation of cerebellar granule cell precursor proliferation and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030214 and hyaluronan catabolic process and biological process this GO :0030324 and lung development and biological process this GO :0030374 and ligand-dependent nuclear receptor transcription coactivator activity and molecular function this GO :0032958 and inositol phosphate biosynthetic process and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042056 and chemoattractant activity and molecular function this GO :0042060 and wound healing and biological process this GO :0042660 and positive regulation of cell fate specification and biological process this GO :0043536 and positive regulation of blood vessel endothelial cell migration and biological process this GO :0043537 and negative regulation of blood vessel endothelial cell migration and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045597 and positive regulation of cell differentiation and biological process this GO :0045669 and positive regulation of osteoblast differentiation and biological process this GO :0045765 and regulation of angiogenesis and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045893 and positive regulation of transcription, this GO :0005104 : fibroblast growth factor receptor binding, this GO :0005104 : fibroblast growth factor receptor binding and also this GO :0005125 : cytokine activity and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity and also this GO :0008201 : heparin binding and also this GO :0030374 : ligand-dependent nuclear receptor transcription coactivator activity and also this GO :0042056 : chemoattractant activity, this GO :0005125 : cytokine activity, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, this GO :0008201 : heparin binding, this GO :0030374 : ligand-dependent nuclear receptor transcription coactivator activity, this GO :0042056 : chemoattractant activity, 66957 and IDBG-630303 and ENSBTAG00000005691 and 281161, fibroblast growth factor 2 (basic) |
| Identity: |
3676 |
| Gene: |
FGF2 |
More about : FGF2 |
| Long gene name: |
fibroblast growth factor 2 |
| Synonyms gene: |
FGFB |
| Synonyms gene name: |
fibroblast growth factor 2 (basic) |
| Locus: |
4q28, 1 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
J04513 |
| Entrez gene record: |
2247 |
| Pubmed identfication: |
9925931 |
| RefSeq identity: |
NM_002006 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000039506 |