| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NKCC1 |
| Specificity: |
This antibody reacts specifically with NKCC1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NKCC1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In mice, In the basolateral membrane of secretory epithelia, Mutations in the gene encoding NCCT cause Gitelmanâ, NCCT is a thiazide-sensitive Na-Cl cotransporter that is primarily expressed in the distal convoluted tubule of the kidney where it accounts for a significant fraction of net renal sodium reabsorption, NKCC1 is expressed in muscle cells, NKCC1 mediates active chloride secretion, NKCC2 is sensitive to the clinically important diuretics furosemide and bumetanide, NKCC2 is specifically expressed in the kidney where it mediates active reabsorption of sodium chloride in the thick ascending limb of the loop of Henle, The gene encoding human NKCC1 maps to chromosome 5q23, The gene encoding human NKCC2 maps to chromosome 15q15-q21 and mutations in this gene lead to Bartterâ, The gene for human NCCT map to chromosome 16q13, a subset of Bartterâ, an inherited hypokalaemic alkalosis, and red blood cells, disruption of the NKCC1 gene leads to deafness and impaired balance, neurons, &euro, &euro, &euro, &trade, &trade, &trade, 3, Na-K-Cl cotransporters (NKCC) are channel proteins that aid in the transcellular movement of chloride across both secretory and absorptive epithelia, s syndrome, s syndrome, s syndrome |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NKCC1 BSC BSC2 PPP1R141 |
| Also known as: |
NKCC1 Polyclonal Antibody |
| Other name: |
Anti-NKCC1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NKCC1 |
| Short name: |
Anti-NKCC1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NKCC1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10911 |
| Gene: |
SLC12A2 |
More about : SLC12A2 |
| Long gene name: |
solute carrier family 12 member 2 |
| Synonyms gene name: |
member 2 , solute carrier family 12 (sodium/potassium/chloride transporter) |
| Synonyms name: |
bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 basolateral Na-K-Cl symporter protein phosphatase 1, regulatory subunit 141 |
| Locus: |
5q23, 3 |
| Discovery year: |
1994-02-16 |
| Entrez gene record: |
6558 |
| Pubmed identfication: |
7629105 |
| RefSeq identity: |
NM_001046 |
| Classification: |
Solute carriers Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000128983 |