| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MYF5 |
| Specificity: |
This antibody reacts specifically with MYF5 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MYF5 |
| Gene ID: |
4617 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
IF2 and HEB, Myf-5 and Myf-6 (also designated MRF-4 or herculin), Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes, Of interest, One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, all turn on expression of myogenin, but when induced to differentiate, most muscle cells express either Myo D or Myf-5 in the committed state, myogenin, the E family, which consists of at least three distinct genes: E2A, Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
bHLHc2 |
| Also known as: |
MYF5 Polyclonal Antibody |
| Other name: |
Anti-MYF5 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MYF5 |
| Short name: |
Anti-MYF5 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MYF5 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7565 |
| Gene: |
MYF5 |
More about : MYF5 |
| Long gene name: |
myogenic factor 5 |
| Locus: |
12q21, 31 |
| Discovery year: |
1989-05-25 |
| Entrez gene record: |
4617 |
| Pubmed identfication: |
8978788 12105204 |
| RefSeq identity: |
NM_005593 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000170166 |