| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Lin-28 |
| Specificity: |
This antibody reacts specifically with Lin-28 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Lin-28B |
| Gene ID: |
79727, 389421 |
| Swiss Prot: |
Q6ZN17, Q9H9Z2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, Isoform 1, Isoform 2 has no effect on cell growth, Mediates MYC-mediated let-7 repression, Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7, Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation, a miRNA precursor, leading to the terminal uridylation of pre-let-7, leading to their terminal uridylation and subsequent degradation, stimulates growth of the breast adenocarcinoma cell line MCF-7, when overexpressed, Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
LIN-28 FLJ12457 ZCCHC1 CSDD1 |
| Also known as: |
Lin-28 Polyclonal Antibody |
| Other name: |
Anti-Lin-28 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Lin-28 |
| Short name: |
Anti-Lin-28 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Lin-28 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
15986 |
| Gene: |
LIN28A |
More about : LIN28A |
| Long gene name: |
lin-28 homolog A |
| Synonyms gene: |
LIN28 |
| Synonyms gene name: |
elegans) , lin-28 homolog (C |
| Locus: |
1p36, 11 |
| Discovery year: |
2003-07-21 |
| GenBank acession: |
AF521099 |
| Entrez gene record: |
79727 |
| Pubmed identfication: |
12798299 |
| RefSeq identity: |
NM_024674 |
| Classification: |
Zinc fingers CCHC-type |
| Havana BLAST/BLAT: |
OTTHUMG00000003550 |