| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MEK1 Thr386 |
| Specificity: |
This antibody reacts specifically with MEK1 (Thr386) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr386 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Thr386 |
| Gene ID: |
5604 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As an essential component of MAP kinase signal transduction pathway, MAP kinases, This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals, act as an integration point for multiple biochemical signals, also known as extracellular signal-regulated kinases (ERKs), differentiation, this kinase is involved in many cellular processes such as proliferation, transcription regulation and development, which acts as a mitogen-activated protein (MAP) kinase kinase, The protein encoded by this gene is a member of the dual specificity protein kinase family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MEK1 MAPKK1 |
| Also known as: |
MEK1 (Thr386) Polyclonal Antibody |
| Other name: |
Anti-MEK1 (Thr386) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MEK1 (Thr386) |
| Short name: |
Anti-MEK1 (Thr386) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MEK1 (Thr386) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6840 |
| Gene: |
MAP2K1 |
More about : MAP2K1 |
| Long gene name: |
mitogen-activated protein kinase kinase 1 |
| Synonyms gene: |
PRKMK1 |
| Locus: |
15q22, 31 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
L11284 |
| Entrez gene record: |
5604 |
| Pubmed identfication: |
9465908 8388392 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000133196 |
| Locus Specific Databases: |
The Globin Gene Server LRG_725 |