| Antigen detectable with this ELISA kit: |
Endothelin 2 (EDN2) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
6, 6, 18, 55, 166, 17-500pg/mL The standard curve concentrations used for the ELISA&rsquo, 17pg/mL, 52pg/mL, 56pg/mL, 67pg/mL, s were 500pg/mL |
| Sensitivity: |
67pg/mL, The minimum detectable dose of this kit is typically less than 2 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
2, 5 hoursours |
| Test principle: |
A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Endothelin 2 (EDN2) and unlabeled Endothelin 2 (EDN2) (Standards or samples) with the pre-coated antibody specific for Endothelin 2 (EDN2), After addition of the substrate solution, After incubation the unbound conjugate is washed off, An antibody specific for Endothelin 2 (EDN2) has been pre-coated onto a microplate, The amount of bound HRP conjugate is reverse proportional to the concentration of Endothelin 2 (EDN2) in the sample, the intensity of color developed is reverse proportional to the concentration of Endothelin 2 (EDN2) in the sample, This assay employs the competitive inhibition enzyme immunoassay technique |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Endothelin 2 (EDN2) |
| Short name: |
Endothelin 2 (EDN2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Endothelin 2 (endothelin 2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
EDN2 and IDBG-640649 and ENSBTAG00000021434 and 319094, EDN2 and IDBG-97144 and ENSG00000127129 and 1907, ET-2 and ET2 and PPET2, Edn2 and IDBG-183488 and ENSMUSG00000028635 and 13615, Extracellular, hormone activity, this GO :0001516 and prostaglandin biosynthetic process and biological process this GO :0001659 and temperature homeostasis and biological process this GO :0002690 and positive regulation of leukocyte chemotaxis and biological process this GO :0003058 and hormonal regulation of the force of heart contraction and biological process this GO :0003099 and positive regulation of the force of heart contraction by chemical signal and biological process this GO :0003100 and regulation of systemic arterial blood pressure by endothelin and biological process this GO :0005179 and hormone activity and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007204 and positive regulation of cytosolic calcium ion concentration and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0010460 and positive regulation of heart rate and biological process this GO :0014824 and artery smooth muscle contraction and biological process this GO :0014826 and vein smooth muscle contraction and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019229 and regulation of vasoconstriction and biological process this GO :0019722 and calcium-mediated signaling and biological process this GO :0030593 and neutrophil chemotaxis and biological process this GO :0042116 and macrophage activation and biological process this GO :0042310 and vasoconstriction and biological process this GO :0045987 and positive regulation of smooth muscle contraction and biological process this GO :0048016 and inositol phosphate-mediated signaling and biological process this GO :0048246 and macrophage chemotaxis and biological process this GO :0048286 and lung alveolus development and biological process this GO :0060585 and positive regulation of prostaglandin-endoperoxide synthase activity and biological process this GO :0097009 and energy homeostasis and biological process, this GO :0005179 : hormone activity, this GO :0005179 : hormone activity, endothelin 2 |
| Identity: |
3177 |
| Gene: |
EDN2 |
More about : EDN2 |
| Long gene name: |
endothelin 2 |
| Synonyms: |
ET2 |
| Locus: |
1p34, 2 |
| Discovery year: |
1989-09-06 |
| GenBank acession: |
M65199 |
| Entrez gene record: |
1907 |
| RefSeq identity: |
NM_001956 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000006362 |