| Antigen detectable with this ELISA kit: |
Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
064ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1), The concentration of Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Endoplasmic Reticulum Nucleus Signalling 1 (ERN1) |
| Short name: |
Endoplasmic Reticulum To Nucleus Signalling 1 (ERN1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Endoplasmic Reticulum To Nucleus Signalling 1 (endoplasmic reticulum to nucleus signaling 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006397 and mRNA processing and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006987 and activation of signaling protein activity involved in unfolded protein response and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0016772 and transferase activity, ERN1 and IDBG-641835 and ENSBTAG00000016799 and 524719, ERN1 and IDBG-64539 and ENSG00000178607 and 2081, Ern1 and IDBG-213476 and ENSMUSG00000020715 and 78943, endonucleolytic and biological process this GO :1900103 and positive regulation of endoplasmic reticulum unfolded protein response and biological process, hIRE1p and IRE1 and IRE1a and IRE1P, nuclei, this GO :0000287 and magnesium ion binding and molecular function this GO :0004519 and endonuclease activity and molecular function this GO :0004521 and endoribonuclease activity and molecular function this GO :0004540 and ribonuclease activity and molecular function this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005637 and nuclear inner membrane and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0006351 and transcription, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004519 : endonuclease activity and also this GO :0004521 : endoribonuclease activity and also this GO :0004540 : ribonuclease activity and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004519 : endonuclease activity, this GO :0004521 : endoribonuclease activity, this GO :0004540 : ribonuclease activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0019899 : enzyme binding, this GO :0042802 : identical protein binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019899 : enzyme binding and also this GO :0042802 : identical protein binding, transferring phosphorus-containing groups and molecular function this GO :0019899 and enzyme binding and molecular function this GO :0030176 and integral component of endoplasmic reticulum membrane and cellular component this GO :0030968 and endoplasmic reticulum unfolded protein response and biological process this GO :0042802 and identical protein binding and molecular function this GO :0044267 and cellular protein metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0070055 and HAC1-type intron splice site recognition and cleavage and biological process this GO :0070059 and intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress and biological process this GO :0090305 and nucleic acid phosphodiester bond hydrolysis and biological process this GO :0090502 and RNA phosphodiester bond hydrolysis, endoplasmic reticulum to nucleus signaling 1 |
| Identity: |
3449 |
| Gene: |
ERN1 |
More about : ERN1 |
| Long gene name: |
endoplasmic reticulum to nucleus signaling 1 |
| Synonyms gene name: |
ER to nucleus signalling 1 |
| Synonyms: |
IRE1 IRE1P |
| Synonyms name: |
inositol-requiring enzyme 1 Serine/threonine-protein kinase/endoribonuclease IRE1 |
| Locus: |
17q23, 3 |
| Discovery year: |
1998-08-06 |
| GenBank acession: |
AF059198 |
| Entrez gene record: |
2081 |
| Pubmed identfication: |
9637683 |
| RefSeq identity: |
NM_001433 |
| Havana BLAST/BLAT: |
OTTHUMG00000178879 |