| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
CHEK1 Ser345 |
| Specificity: |
This antibody reacts specifically with CHEK1 (Ser345) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser345 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser345 |
| Gene ID: |
1111 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA, Oct 2011], Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks, Several alternatively spliced transcript variants have been found for this gene, This protein acts to integrate signals from ATM and ATR, [provided by RefSeq, that also associate with chromatin in meiotic prophase I, two cell cycle proteins involved in DNA damage responses, The protein encoded by this gene belongs to the Ser/Thr protein kinase family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CHK1 |
| Also known as: |
CHEK1 (Ser345) Polyclonal Antibody |
| Other name: |
Anti-CHEK1 (Ser345) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
CHEK1 (Ser345) |
| Short name: |
Anti-CHEK1 (Ser345) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-CHEK1 (Ser345) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1925 |
| Gene: |
CHEK1 |
More about : CHEK1 |
| Long gene name: |
checkpoint kinase 1 |
| Synonyms gene name: |
CHK1 (checkpoint, S, pombe) , pombe) homolog CHK1 checkpoint homolog (S |
| Locus: |
11q24, 2 |
| Discovery year: |
1998-04-21 |
| GenBank acession: |
AF016582 BC017575 |
| Entrez gene record: |
1111 |
| Pubmed identfication: |
9278511 9382850 |
| RefSeq identity: |
NM_001274 |
| Havana BLAST/BLAT: |
OTTHUMG00000165853 |