| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
AKT3 (Ser472) |
| Specificity: |
AKT3 (Ser472), This antibody will cross react with Akt1 when phosphorylated at Ser473 and Akt2 when phosphorylated at Ser474 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser472 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human AKT3 around the phosphorylation site of Ser472 |
| Gene ID: |
10000 |
| Swiss Prot: |
Q9Y243 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
AKT2 and AKT3) called the AKT kinase, AKT3 is the least studied AKT isoform, AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13, Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, It plays an important role in brain development and is crucial for the viability of malignant glioma cells, Over 100 substrate candidates have been reported so far, Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands, This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates, and which regulate many processes including metabolism, but for most of them, cell survival, growth and angiogenesis, no isoform specificity has been reported, proliferation, resulting in the induction of caspase-dependent apoptosis, AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PKBG RAC-gamma PRKBG |
| Also known as: |
AKT3 (Ser472) Polyclonal Antibody |
| Other name: |
Anti-AKT3 (Ser472) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
AKT3 (Ser472) |
| Short name: |
Anti-AKT3 (Ser472) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-AKT3 (Ser472) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
393 |
| Gene: |
AKT3 |
More about : AKT3 |
| Long gene name: |
AKT serine/threonine kinase 3 |
| Synonyms gene name: |
v-akt murine thymoma viral oncogene homolog 3 |
| Synonyms name: |
gamma , protein kinase B |
| Locus: |
1q43-q44 |
| Discovery year: |
1999-11-16 |
| GenBank acession: |
AF124141 |
| Entrez gene record: |
10000 |
| Pubmed identfication: |
10092583 10208883 |
| RefSeq identity: |
NM_181690 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000039994 |