| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Rb2 p130 |
| Specificity: |
This antibody reacts specifically with Rb2 p130 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Rb2 p130 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding of p130 converts E2F transcription factors from transcriptional activators to transcriptional repressors, E2F transcription factors regulate the expression of a number of genes important in cell proliferation, particularly those involved in the progression through G1 and into the S phase of the cell cycle, p130 is related both in structure and function to the retinoblastoma tumor suppressor protein and p107 (collectively known as pocket proteins) and is known to regulate the activity of E2F transcription factors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Rb2 p130 |
| Also known as: |
Rb2 p130 Polyclonal Antibody |
| Other name: |
Anti-Rb2 p130 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Rb2 p130 |
| Short name: |
Anti-Rb2 p130 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Rb2 p130 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9894 |
| Gene: |
RBL2 |
More about : RBL2 |
| Long gene name: |
RB transcriptional corepressor like 2 |
| Synonyms gene name: |
retinoblastoma-like 2 |
| Locus: |
16q12, 2 |
| Discovery year: |
1994-01-17 |
| GenBank acession: |
X74594 |
| Entrez gene record: |
5934 |
| Pubmed identfication: |
8361765 8643454 |
| RefSeq identity: |
NM_005611 |
| Havana BLAST/BLAT: |
OTTHUMG00000133198 |