| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
H1N1 Nucleoprotein |
| Specificity: |
This antibody reacts specifically with H1N1 Nucleoprotein |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from Influenza A virus (strain A/Russia:St, Petersburg/8/2006 H1N1) Nucleoprotein |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Virus |
| Cross Reactive Species details: |
Influenza A virus |
| Background information: |
Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export, Later in the infection, M1 dissociates from the RNP under acidification of the virion driven by M2 protein, M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals, NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway, Soon after a virion infects a new cell, The RNP needs to be localized in the nucleus to start an infectious cycle, The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication, but is too large to diffuse through the nuclear pore complex, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins, protecting it from nucleases, targeting the RNP to the nucleus, Encapsidates the negative strand viral RNA |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
H2N2 Nucleoprotein, H3N2 Nucleoprotein, H3N8 Nucleoprotein, H5N1 Nucleoprotein, H7N7 Nucleoprotein, H9N2 Nucleoprotein, Influenza A virus H1N1, NP, Nucleocapsid protein, Protein N, Nucleoprotein |
| Also known as: |
H1N1 Nucleoprotein Polyclonal Antibody |
| Other name: |
Anti-H1N1 Nucleoprotein Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
H1N1 Nucleoprotein |
| Short name: |
Anti-H1N1 Nucleoprotein (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-H1N1 Nucleoprotein (polyclonal) |
| Alternative technique: |
polyclonals |