| Antigen detectable with this ELISA kit: |
Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
062ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), The concentration of Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) |
| Short name: |
Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ENPP1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Ectonucleotide Pyrophosphatase/Phosphodiesterase 1 (ectonucleotide pyrophosphatase/phosphodiesterase 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
3'-phosphoadenosine 5'-phosphosulfate binding, ARHR2 and COLED and M6S1 and NPP1 and NPPS and PC-1 and PCA1 and PDNP1, ENPP1 and IDBG-633303 and ENSBTAG00000021830 and 615535, ENPP1 and IDBG-96591 and ENSG00000197594 and 5167, Enpp1 and IDBG-138688 and ENSMUSG00000037370 and 18605, Extracellular, this GO :0003676 and nucleic acid binding and molecular function this GO :0003824 and catalytic activity and molecular function this GO :0004528 and phosphodiesterase I activity and molecular function this GO :0004551 and nucleotide diphosphatase activity and molecular function this GO :0005044 and scavenger receptor activity and molecular function this GO :0005158 and insulin receptor binding and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005765 and lysosomal membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006091 and generation of precursor metabolites and energy and biological process this GO :0006200 and ATP catabolic process and biological process this GO :0006766 and vitamin metabolic process and biological process this GO :0006767 and water-soluble vitamin metabolic process and biological process this GO :0006771 and riboflavin metabolic process and biological process this GO :0006796 and phosphate-containing compound metabolic process and biological process this GO :0006898 and receptor-mediated endocytosis and biological process this GO :0006955 and immune response and biological process this GO :0008152 and metabolic process and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0009143 and nucleoside triphosphate catabolic process and biological process this GO :0009986 and cell surface and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016323 and basolateral plasma membrane and cellular component this GO :0016787 and hydrolase activity and molecular function this GO :0030247 and polysaccharide binding and molecular function this GO :0030279 and negative regulation of ossification and biological process this GO :0030308 and negative regulation of cell growth and biological process this GO :0030500 and regulation of bone mineralization and biological process this GO :0030505 and inorganic diphosphate transport and biological process this GO :0030643 and cellular phosphate ion homeostasis and biological process this GO :0030730 and sequestering of triglyceride and biological process this GO :0031214 and biomineral tissue development and biological process this GO :0031953 and negative regulation of protein autophosphorylation and biological process this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0035529 and NADH pyrophosphatase activity and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0044281 and small molecule metabolic process and biological process this GO :0045599 and negative regulation of fat cell differentiation and biological process this GO :0045719 and negative regulation of glycogen biosynthetic process and biological process this GO :0046325 and negative regulation of glucose import and biological process this GO :0046627 and negative regulation of insulin receptor signaling pathway and biological process this GO :0046849 and bone remodeling and biological process this GO :0046872 and metal ion binding and molecular function this GO :0047429 and nucleoside-triphosphate diphosphatase activity and molecular function this GO :0050427 and 3'-phosphoadenosine 5'-phosphosulfate metabolic process and biological process this GO :0050656 and 3'-phosphoadenosine 5'-phosphosulfate binding and molecular function this GO :0090305 and nucleic acid phosphodiester bond hydrolysis and biological process, this GO :0003676 : nucleic acid binding, this GO :0003676 : nucleic acid binding and also this GO :0003824 : catalytic activity and also this GO :0004528 : phosphodiesterase I activity and also this GO :0004551 : nucleotide diphosphatase activity and also this GO :0005044 : scavenger receptor activity and also this GO :0005158 : insulin receptor binding and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008270 : zinc ion binding and also this GO :0016787 : hydrolase activity and also this GO :0030247 : polysaccharide binding and also this GO :0035529 : NADH pyrophosphatase activity and also this GO :0042803 : protein homodimerization activity and also this GO :0046872 : metal ion binding and also this GO :0047429 : nucleoside-triphosphate diphosphatase activity and also this GO :0050656 : 3'-phosphoadenosine 5'-phosphosulfate binding, this GO :0003824 : catalytic activity, this GO :0004528 : phosphodiesterase I activity, this GO :0004551 : nucleotide diphosphatase activity, this GO :0005044 : scavenger receptor activity, this GO :0005158 : insulin receptor binding, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008270 : zinc ion binding, this GO :0016787 : hydrolase activity, this GO :0030247 : polysaccharide binding, this GO :0035529 : NADH pyrophosphatase activity, this GO :0042803 : protein homodimerization activity, this GO :0046872 : metal ion binding, this GO :0047429 : nucleoside-triphosphate diphosphatase activity, this GO :0050656 : 3'-phosphoadenosine 5'-phosphosulfate binding, ectonucleotide pyrophosphatase/phosphodiesterase 1 |
| Identity: |
3356 |
| Gene: |
ENPP1 |
More about : ENPP1 |
| Long gene name: |
ectonucleotide pyrophosphatase/phosphodiesterase 1 |
| Synonyms gene: |
NPPS M6S1 PDNP1 |
| Synonyms: |
PC-1 PCA1 |
| Locus: |
6q23, 2 |
| Discovery year: |
1992-12-08 |
| GenBank acession: |
M57736 |
| Entrez gene record: |
5167 |
| Pubmed identfication: |
1315502 |
| Havana BLAST/BLAT: |
OTTHUMG00000015572 |