| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PDIA2 |
| Specificity: |
This antibody reacts specifically with PDIA2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PDIA2 |
| Gene ID: |
64714 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
PDI, Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively), The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER, This retention is reported to be mediated by a KDEL receptor, as well as the reduction of disulphide bonds in proteins, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds, which catalyses disulphide interchange between thiols and protein dilsulphides, The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PDA2 PDI PDIR |
| Also known as: |
PDIA2 Polyclonal Antibody |
| Other name: |
Anti-PDIA2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PDIA2 |
| Short name: |
Anti-PDIA2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, member 2 (polyclonal), antibody to-protein disulfide isomerase family A |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, PDIA2 and IDBG-7384 and ENSG00000185615 and 64714, Pdia2 and IDBG-154856 and ENSMUSG00000024184 and 69191, member 2, multiple, peptidyl-proline 4-dioxygenase activity, this GO :0003756 and protein disulfide isomerase activity and molecular function this GO :0005496 and steroid binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0006457 and protein folding and biological process this GO :0006621 and protein retention in ER lumen and biological process this GO :0008152 and metabolic process and biological process this GO :0016209 and antioxidant activity and molecular function this GO :0016491 and oxidoreductase activity and molecular function this GO :0016853 and isomerase activity and molecular function this GO :0019511 and peptidyl-proline hydroxylation and biological process this GO :0031545 and peptidyl-proline 4-dioxygenase activity and molecular function this GO :0034976 and response to endoplasmic reticulum stress and biological process this GO :0045454 and cell redox homeostasis and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :1902175 and regulation of oxidative stress-induced intrinsic apoptotic signaling pathway and biological process, this GO :0003756 : protein disulfide isomerase activity, this GO :0003756 : protein disulfide isomerase activity and also this GO :0005496 : steroid binding and also this GO :0005515 : protein binding and also this GO :0016209 : antioxidant activity and also this GO :0016491 : oxidoreductase activity and also this GO :0016853 : isomerase activity and also this GO :0031545 : peptidyl-proline 4-dioxygenase activity, this GO :0005496 : steroid binding, this GO :0005515 : protein binding, this GO :0016209 : antioxidant activity, this GO :0016491 : oxidoreductase activity, this GO :0016853 : isomerase activity, this GO :0031545 : peptidyl-proline 4-dioxygenase activity, 51496 and IDBG-633666 and ENSBTAG00000016575 and 504356, protein disulfide isomerase family A |
| Identity: |
14180 |
| Gene: |
PDIA2 |
More about : PDIA2 |
| Long gene name: |
protein disulfide isomerase family A member 2 |
| Synonyms gene: |
PDIP |
| Synonyms gene name: |
member 2 , pancreatic protein disulfide isomerase-associated 2 protein disulfide isomerase family A, protein disulfide isomerase |
| Locus: |
16p13, 3 |
| Discovery year: |
2000-12-21 |
| GenBank acession: |
U19948 |
| Entrez gene record: |
64714 |
| Pubmed identfication: |
8561901 |
| RefSeq identity: |
NM_006849 |
| Classification: |
Protein disulfide isomerases |
| Havana BLAST/BLAT: |
OTTHUMG00000064891 |