| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Keratan Sulfate |
| Specificity: |
This antibody reacts specifically with Keratan Sulfate |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Keratan Sulfate |
| Gene ID: |
8534 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Aggrecan is the major proteoglycan of human articular cartilage, It also may function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins, It has a preference for sulfating keratan sulfate, It participates in biosynthesis of selectin ligands, Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation, The core protein is substituted by a number of keratan sulfate and chondroitin sulfate glycosaminoglycan chains, The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, Whereas chondroitin sulfate is widely distributed throughout the body, and only internal galactose residues are sulfated, but it also transfers sulfate to the unsulfated polymer, intervertebral discs) and cornea, keratan sulfate is primarily expressed in cartilage (joints, trachea, Catalyzes the transfer of sulfate to position 6 of galactose residues of keratan |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
C6ST KSGal6ST |
| Also known as: |
Keratan Sulfate Polyclonal Antibody |
| Other name: |
Anti-Keratan Sulfate Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Keratan Sulfate |
| Short name: |
Anti-Keratan Sulfate (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Keratan Sulfate (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
1969 |
| Gene: |
CHST1 |
More about : CHST1 |
| Long gene name: |
carbohydrate sulfotransferase 1 |
| Synonyms gene name: |
carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 |
| Synonyms name: |
Keratan sulfotransferase |
| Locus: |
11p11, 2 |
| Discovery year: |
1998-10-02 |
| GenBank acession: |
U65637 |
| Entrez gene record: |
8534 |
| Pubmed identfication: |
9405439 9639683 |
| RefSeq identity: |
NM_003654 |
| Classification: |
Sulfotransferases, membrane bound |
| Havana BLAST/BLAT: |
OTTHUMG00000166513 |