| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PDHA1 |
| Specificity: |
This antibody reacts specifically with PDHA1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Mitochondrion |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PDHA1 |
| Gene ID: |
5160 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Alternatively spliced transcript variants encoding different isoforms have been found for this gene, Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome, The E1 enzyme is a heterotetramer of two alpha and two beta subunits, The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase(E3), The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
PDHA1 Polyclonal Antibody |
| Other name: |
Anti-PDHA1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PDHA1 |
| Short name: |
Anti-PDHA1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PDHA1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
8806 |
| Gene: |
PDHA1 |
More about : PDHA1 |
| Long gene name: |
pyruvate dehydrogenase (lipoamide) alpha 1 |
| Synonyms gene: |
PDHA |
| Synonyms name: |
mitochondrial , pyruvate dehydrogenase E1 component subunit alpha, somatic form |
| Locus: |
Xp22, 12 |
| Discovery year: |
1989-06-30 |
| Entrez gene record: |
5160 |
| Havana BLAST/BLAT: |
OTTHUMG00000021224 |
| Locus Specific Databases: |
Mental Retardation database |