| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HK2/Hexokinase 2 |
| Specificity: |
This antibody reacts specifically with HK2/Hexokinase 2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Hexokinase 2 |
| Gene ID: |
3099 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Four hexokinase isoenzymes have been identified, Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, Glucokinase lacks the N-terminal hydrophobic cluster, HXK I has been shown to be expressed in brain, HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues, HXK III has the highest affinity for glucose, Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids, Of the hexokinases, The hydrophobic cluster is thought to be necessary for membrane binding, This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases, also designated glucokinase or GCK), determining the â, hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, including hexokinase I (HXK I), kidney and heart tissues as well as in hepatoma cell lines, the conversion of glucose to glucose-6-phosphate, &euro, &euro, &oelig, The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, set pointâ, for insulin secretion |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
HK2/Hexokinase 2 Polyclonal Antibody |
| Other name: |
Anti-HK2/Hexokinase 2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HK2/Hexokinase 2 |
| Short name: |
Anti-HK2/Hexokinase 2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HK2/Hexokinase 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4923 |
| Gene: |
HK2 |
More about : HK2 |
| Long gene name: |
hexokinase 2 |
| Locus: |
2p12 |
| Discovery year: |
1993-03-11 |
| Entrez gene record: |
3099 |
| RefSeq identity: |
NM_000189 |
| Havana BLAST/BLAT: |
OTTHUMG00000129972 |