| Antigen detectable with this ELISA kit: |
Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
113ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2), The concentration of Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dimethylarginine Dimethylaminohydrolase 2 (DDAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Dimethylarginine Dimethylaminohydrolase 2 (DDAH2), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) |
| Short name: |
Dimethylarginine Dimethylaminohydrolase 2 (DDAH2) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Dimethylarginine Dimethylaminohydrolase 2 (dimethylarginine dimethylaminohydrolase 2) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
Cytoplasm, DDAH and DDAHII and G6a and HEL-S-277 and NG30, DDAH2 and IDBG-238590 and ENSG00000213722 and 23564, DDAH2 and IDBG-633979 and ENSBTAG00000013530 and 540386, Ddah2 and IDBG-176274 and ENSMUSG00000007039 and 51793, acting on carbon-nitrogen (but not peptide) bonds, acting on carbon-nitrogen (but not peptide) bonds, acting on carbon-nitrogen (but not peptide) bonds, hydrolase activity, in linear amidines, in linear amidines, in linear amidines and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0000052 and citrulline metabolic process and biological process this GO :0003824 and catalytic activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0006525 and arginine metabolic process and biological process this GO :0006527 and arginine catabolic process and biological process this GO :0006809 and nitric oxide biosynthetic process and biological process this GO :0007263 and nitric oxide mediated signal transduction and biological process this GO :0016403 and dimethylargininase activity and molecular function this GO :0016597 and amino acid binding and molecular function this GO :0016813 and hydrolase activity, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0005515 : protein binding and also this GO :0016403 : dimethylargininase activity and also this GO :0016597 : amino acid binding and also this GO :0016813 : hydrolase activity, this GO :0005515 : protein binding, this GO :0016403 : dimethylargininase activity, this GO :0016597 : amino acid binding, this GO :0016813 : hydrolase activity, dimethylarginine dimethylaminohydrolase 2 |
| Identity: |
2716 |
| Gene: |
DDAH2 |
More about : DDAH2 |
| Long gene name: |
dimethylarginine dimethylaminohydrolase 2 |
| Synonyms: |
G6A |
| Locus: |
6p21, 33 |
| Discovery year: |
1999-10-22 |
| GenBank acession: |
AF070667 |
| Entrez gene record: |
23564 |
| Pubmed identfication: |
10493931 |
| Havana BLAST/BLAT: |
OTTHUMG00000031212 |