| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Villin |
| Specificity: |
This antibody reacts specifically with Villin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Villin |
| Gene ID: |
7429 |
| Swiss Prot: |
P09327 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Appears to regulate cell death by maintaining mitochondrial integrity, Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities, Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity, Binds phosphatidylinositol 4, Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, Plays a role in the actin nucleation, Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium, Regulates the intestinal epithelial cell morphology, Upon S, actin filament bundle assembly, actin filament capping and severing, binds LPA with higher affinity than PIP2, cell invasion, cell migration and apoptosis, chemotaxis and wound repair, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination, 5-bisphosphate (PIP2) and lysophosphatidic acid (LPA), Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments, flexneri cell infection |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Villin Polyclonal Antibody |
| Other name: |
Anti-Villin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Villin |
| Short name: |
Anti-Villin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Villin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
30906 |
| Gene: |
VILL |
More about : VILL |
| Long gene name: |
villin like |
| Locus: |
3p22, 2 |
| Discovery year: |
2004-07-28 |
| Entrez gene record: |
50853 |
| Pubmed identfication: |
9179494 |
| RefSeq identity: |
NM_015873 |
| Classification: |
Gelsolin/villins |
| Havana BLAST/BLAT: |
OTTHUMG00000130814 |