| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HSF1 |
| Specificity: |
This antibody reacts specifically with HSF1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HSF1 |
| Gene ID: |
3297 |
| Swiss Prot: |
Q00613 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
HSF is unable to bind to the HSE unless the cells are heat shocked, In higher eukaryotes, DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HSTF1 |
| Also known as: |
HSF1 Polyclonal Antibody |
| Other name: |
Anti-HSF1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HSF1 |
| Short name: |
Anti-HSF1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-heat shock transcription factor 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0006952 and defense response and biological process this GO :0007143 and female meiotic division and biological process this GO :0007283 and spermatogenesis and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0009408 and response to heat and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0034605 and cellular response to heat and biological process this GO :0040018 and positive regulation of multicellular organism growth and biological process this GO :0043234 and protein complex and cellular component this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045120 and pronucleus and cellular component this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0060136 and embryonic process involved in female pregnancy and biological process this GO :0090231 and regulation of spindle checkpoint and biological process, HSF1 and IDBG-40498 and ENSG00000185122 and 3297, HSF1 and IDBG-643592 and ENSBTAG00000020751 and 506235, HSTF1, Hsf1 and IDBG-155311 and ENSMUSG00000022556 and 15499, nuclei, sequence-specific DNA binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001162 and RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and molecular function this GO :0001701 and in utero embryonic development and biological process this GO :0001892 and embryonic placenta development and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006351 and transcription, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0001162 : RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0043565 : sequence-specific DNA binding, this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0001162 : RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0043565 : sequence-specific DNA binding, heat shock transcription factor 1 |
| Identity: |
5224 |
| Gene: |
HSF1 |
More about : HSF1 |
| Long gene name: |
heat shock transcription factor 1 |
| Locus: |
8q24, 3 |
| Discovery year: |
1994-09-12 |
| GenBank acession: |
M64673 |
| Entrez gene record: |
3297 |
| Pubmed identfication: |
1871105 |
| RefSeq identity: |
NM_005526 |
| Havana BLAST/BLAT: |
OTTHUMG00000174604 |