| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PKC gamma/SCA14 |
| Specificity: |
This antibody reacts specifically with PKC gamma/SCA14 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PKC gamma/SCA14 |
| Gene ID: |
5582 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14), Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells, It has been demonstrated that several neuronal functions, Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development, PKC also serve as major receptors for phorbol esters, PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways, PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons, The protein encoded by this gene is one of the PKC family members, There have been at least 12 different PKC isoforms identified in humans to date including alpha, a class of tumor promoters, and mu, beta I, beta II, delta, epsilon, eta, gamma, including long term potentiation (LTP) and long term depression (LTD), iota, lambda, specifically require this kinase, theta, zeta, Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PKCC MGC57564 |
| Also known as: |
PKC gamma/SCA14 Polyclonal Antibody |
| Other name: |
Anti-PKC gamma/SCA14 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PKC gamma/SCA14 |
| Short name: |
Anti-PKC gamma/SCA14 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PKC g/SCA14 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9402 |
| Gene: |
PRKCG |
More about : PRKCG |
| Long gene name: |
protein kinase C gamma |
| Synonyms gene: |
PKCG SCA14 |
| Synonyms gene name: |
gamma , protein kinase C |
| Synonyms name: |
PKC-gamma |
| Locus: |
19q13, 42 |
| Discovery year: |
1991-08-02 |
| GenBank acession: |
M13977 |
| Entrez gene record: |
5582 |
| Pubmed identfication: |
8432525 3755548 |
| RefSeq identity: |
NM_002739 |
| Classification: |
C2 domain containing protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000064846 |
| Locus Specific Databases: |
LRG_669 , Mutations of the Protein Kinase C &, #947 |