| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SIAH1 |
| Specificity: |
This antibody reacts specifically with SIAH1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SIAH1 |
| Gene ID: |
6477 |
| Swiss Prot: |
Q8IUQ4 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Confers constitutive instability to HIPK2 through proteasomal degradation, E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, GAPDH acts as a stabilizer of SIAH1, Has some overlapping function with SIAH2, Induces apoptosis in cooperation with PEG3, It is thereby involved in many cellular processes such as apoptosis, MYB, Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes, PML and RBBP8), POU2AF1, Triggers the ubiquitin-mediated degradation of many substrates, Upon nitric oxid (NO) generation that follows apoptotic stimulation, a cell surface receptor (DCC), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP, an antiapoptotic protein (BAG1), axon guidance, cell cycle, facilitating the degradation of nuclear proteins, including proteins involved in transcription regulation (ELL2, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus, spermatogenesis and TNF-alpha signaling, the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), transcription regulation, tumor suppression, E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
hSIAH1 |
| Also known as: |
SIAH1 Polyclonal Antibody |
| Other name: |
Anti-SIAH1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SIAH1 |
| Short name: |
Anti-SIAH1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-siah E3 ubiquitin protein ligase 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, SIAH1 and IDBG-30112 and ENSG00000196470 and 6477, SIAH1A, ligase activity, nuclei, this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005769 and early endosome and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006508 and proteolysis and biological process this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0007049 and cell cycle and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007399 and nervous system development and biological process this GO :0007411 and axon guidance and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0016874 and ligase activity and molecular function this GO :0030163 and protein catabolic process and biological process this GO :0030877 and beta-catenin destruction complex and cellular component this GO :0031648 and protein destabilization and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0051402 and neuron apoptotic process and biological process this GO :2001244 and positive regulation of intrinsic apoptotic signaling pathway and biological process, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0008022 : protein C-terminus binding and also this GO :0008270 : zinc ion binding and also this GO :0016874 : ligase activity, this GO :0005515 : protein binding, this GO :0008022 : protein C-terminus binding, this GO :0008270 : zinc ion binding, this GO :0016874 : ligase activity, 37798 and IDBG-631496 and ENSBTAG00000010868 and 534655, siah E3 ubiquitin protein ligase 1 |
| Identity: |
10857 |
| Gene: |
SIAH1 |
More about : SIAH1 |
| Long gene name: |
siah E3 ubiquitin protein ligase 1 |
| Synonyms gene name: |
seven in absentia homolog 1 (Drosophila) |
| Locus: |
16q12, 1 |
| Discovery year: |
1997-09-12 |
| GenBank acession: |
U76247 |
| Entrez gene record: |
6477 |
| Pubmed identfication: |
9403064 9334332 |
| Havana BLAST/BLAT: |
OTTHUMG00000175417 |