| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MAP3K8 |
| Specificity: |
This antibody reacts specifically with MAP3K8 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MAP3K8 |
| Gene ID: |
1326 |
| Swiss Prot: |
P41279 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In adipocytes, In vitro, Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production, Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells, Isoform 1 shows some transforming activity, May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types, Plays a role in the cell cycle, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, although it is much weaker than that of the activated oncogenic variant, and thus may play a role in the regulation of immunoglobulin production, but not insulin, leading to induction of lipolysis, leading to up-regulation of IL8 and CCL4, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses, Required for lipopolysaccharide (LPS)-induced |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Tpl-2 EST c-COT MEKK8 |
| Also known as: |
MAP3K8 Polyclonal Antibody |
| Other name: |
Anti-MAP3K8 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MAP3K8 |
| Short name: |
Anti-MAP3K8 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MAP3K8 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6860 |
| Gene: |
MAP3K8 |
More about : MAP3K8 |
| Long gene name: |
mitogen-activated protein kinase kinase kinase 8 |
| Synonyms gene: |
COT ESTF |
| Locus: |
10p11, 23 |
| Discovery year: |
1992-09-14 |
| GenBank acession: |
D14497 |
| Entrez gene record: |
1326 |
| Pubmed identfication: |
2072910 8479752 |
| RefSeq identity: |
NM_005204 |
| Classification: |
Mitogen-activated protein kinase kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000017889 |