| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Tuberin |
| Specificity: |
This antibody reacts specifically with Tuberin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Tuberin |
| Gene ID: |
7249 |
| Swiss Prot: |
P49815 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, Implicated as a tumor suppressor, Involved in microtubule-mediated protein transport, Mutations in TSC2 lead to constitutive activation of RAP1A in tumors, Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro, a direct activator of the protein kinase activity of mTORC1, but this seems to be due to unregulated mTOR signaling, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling, In complex with TSC1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
tuberin LAM PPP1R160 TSC2 |
| Also known as: |
Tuberin Polyclonal Antibody |
| Other name: |
Anti-Tuberin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Tuberin |
| Short name: |
Anti-Tuberin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Tuberin (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12365 |
| Gene: |
TSC4 |
More about : TSC4 |
| Long gene name: |
tuberous sclerosis 2 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 160 |
| Locus: |
16p13, 3 |
| Discovery year: |
1989-05-25 |
| GenBank acession: |
AB014460 |
| Entrez gene record: |
7249 |
| Pubmed identfication: |
1303246 7558029 |
| RefSeq identity: |
NM_000548 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000128745 |
| Locus Specific Databases: |
TSC2 Mutation Database Tuberous sclerosis database LRG_487 |