| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RASGRF1 Ser916 |
| Specificity: |
This antibody reacts specifically with RASGRF1 (Ser916) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser916 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from mouse RasGRF1 around the phosphorylation site of Ser916 |
| Gene ID: |
5923 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Alternatively spliced transcript variants encoding distinct isoforms have been reported, Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein, Mar 2009], Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory, The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, [provided by RefSeq, and G protein beta-gamma subunit, muscarinic receptors, The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CDC25L CDC25 GRF55 H-GRF55 GNRP PP13187 |
| Also known as: |
RASGRF1 (Ser916) Polyclonal Antibody |
| Other name: |
Anti-RASGRF1 (Ser916) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RASGRF1 (Ser916) |
| Short name: |
Anti-RASGRF1 (Ser916) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Ras protein-specific guanine nucleotide-releasing factor 1 (Ser916) (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
CDC25 and CDC25L and GNRP and GRF1 and GRF55 and H-GRF55 and PP13187 and ras-GRF1, Plasma membranes, RASGRF1 and IDBG-25346 and ENSG00000058335 and 5923, RASGRF1 and IDBG-630181 and ENSBTAG00000019940 and 520039, Rasgrf1 and IDBG-190479 and ENSMUSG00000032356 and 19417, glutamate receptor binding, this GO :0005085 and guanyl-nucleotide exchange factor activity and molecular function this GO :0005088 and Ras guanyl-nucleotide exchange factor activity and molecular function this GO :0005089 and Rho guanyl-nucleotide exchange factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007165 and signal transduction and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007268 and synaptic transmission and biological process this GO :0007616 and long-term memory and biological process this GO :0008283 and cell proliferation and biological process this GO :0030426 and growth cone and cellular component this GO :0031175 and neuron projection development and biological process this GO :0032320 and positive regulation of Ras GTPase activity and biological process this GO :0032863 and activation of Rac GTPase activity and biological process this GO :0035020 and regulation of Rac protein signal transduction and biological process this GO :0035023 and regulation of Rho protein signal transduction and biological process this GO :0035254 and glutamate receptor binding and molecular function this GO :0043005 and neuron projection and cellular component this GO :0046578 and regulation of Ras protein signal transduction and biological process this GO :0046579 and positive regulation of Ras protein signal transduction and biological process this GO :0048167 and regulation of synaptic plasticity and biological process this GO :0048168 and regulation of neuronal synaptic plasticity and biological process this GO :0051056 and regulation of small GTPase mediated signal transduction and biological process this GO :2000310 and regulation of N-methyl-D-aspartate selective glutamate receptor activity and biological process, this GO :0005085 : guanyl-nucleotide exchange factor activity, this GO :0005085 : guanyl-nucleotide exchange factor activity and also this GO :0005088 : Ras guanyl-nucleotide exchange factor activity and also this GO :0005089 : Rho guanyl-nucleotide exchange factor activity and also this GO :0005515 : protein binding and also this GO :0035254 : glutamate receptor binding, this GO :0005088 : Ras guanyl-nucleotide exchange factor activity, this GO :0005089 : Rho guanyl-nucleotide exchange factor activity, this GO :0005515 : protein binding, this GO :0035254 : glutamate receptor binding, Ras protein-specific guanine nucleotide-releasing factor 1 |
| Identity: |
9875 |
| Gene: |
RASGRF1 |
More about : RASGRF1 |
| Long gene name: |
Ras protein specific guanine nucleotide releasing factor 1 |
| Synonyms gene: |
GRF1 |
| Locus: |
15q25, 1 |
| Discovery year: |
1994-02-11 |
| GenBank acession: |
M91815 |
| Entrez gene record: |
5923 |
| Pubmed identfication: |
7684828 1379731 |
| RefSeq identity: |
NM_002891 |
| Classification: |
Rho guanine nucleotide exchange factors Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000144172 |