| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PAR4 Thr163 |
| Specificity: |
This antibody reacts specifically with PAR4 (Thr163) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr163 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human PAR4 around the phosphorylation site of Thr163 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
2001), 2001), 2006), However, Mice lacking PAR4 are protected from mesenteric arteriole thrombosis, PAR1 and PAR3 are activated by thrombin, PAR4 is expressed along with PAR1 on human platelets, The PAR family consists of 4 receptors, and PAR2 and PAR4 are activated by several serine proteases (Macfarlane et al, and each plays an important role in thrombin-induced platelet aggregation (Holinstat et al, and platelets from mice lacking PAR4 fail to aggregate in response to thrombin (Sambrano et al, indicating that PAR4 is a potential target for treatment of thrombosis in humans, platelets from mice express PAR4 but not PAR1, that are activated by proteolytic cleavage of the N-terminal domain of the receptor to reveal a tethered ligand, the proteinase-activated receptors (PARs), PAR4 is a member of a unique family of GPCRs |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PAR4 PAR-4 |
| Also known as: |
PAR4 (Thr163) Polyclonal Antibody |
| Other name: |
Anti-PAR4 (Thr163) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PAR4 (Thr163) |
| Short name: |
Anti-PAR4 (Thr163) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PAR4 (Thr163) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
29998 |
| Gene: |
PWAR4 |
More about : PWAR4 |
| Long gene name: |
Prader Willi/Angelman region RNA 4 |
| Locus: |
15q11, 2 |
| Discovery year: |
2013-06-14 |
| Entrez gene record: |
347745 |
| Pubmed identfication: |
11106375 7987392 |