| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NuMA Ser395 |
| Specificity: |
This antibody reacts specifically with NuMA (Ser395) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser395 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human NuMA around the phosphorylation site of Ser395 |
| Gene ID: |
4926 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
After anaphase, At the onset of mitosis, NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly, Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole, the protein redistributes from the spindle polar region into reforming nucleus, NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Centrophilin stabilizes mitotic spindle in mitotic cells, NUMA1, NuMA protein, Nuclear mitotic apparatus protein, Nuclear mitotic apparatus protein 1, SP H antigen, Structural nuclear protein, NUMA 1 |
| Also known as: |
NuMA (Ser395) Polyclonal Antibody |
| Other name: |
Anti-NuMA (Ser395) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NuMA (Ser395) |
| Short name: |
Anti-NuMA (Ser395) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NuMA (Ser395) (polyclonal) |
| Alternative technique: |
polyclonals |