| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
IRAK1 Thr387 |
| Specificity: |
This antibody reacts specifically with IRAK1 (Thr387) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr387 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr387 [RG(p-T)LA] |
| Gene ID: |
3654 |
| Swiss Prot: |
P51617 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Alternatively, Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation, In turn, Involved in Toll-like receptor (TLR) and IL-1R signaling pathways, Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1, Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, Then, When sumoylated, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation, resulting in transcriptional activation of type I IFN genes, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex, translocates to the nucleus and phosphorylates STAT3, which drive the cell in an antiviral state, Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
IRAK pelle |
| Also known as: |
IRAK1 (Thr387) Polyclonal Antibody |
| Other name: |
Anti-IRAK1 (Thr387) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IRAK1 (Thr387) |
| Short name: |
Anti-IRAK1 (Thr387) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-interleukin-1 receptor-associated kinase 1 (Thr387) (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051259 and protein oli this GO merization and biological process this GO :0051403 and stress-activated MAPK cascade and biological process this GO :0070423 and nucleotide-binding oli this GO merization domain containing signaling pathway and biological process this GO :0070498 and interleukin-1-mediated signaling pathway and biological process this GO :0070555 and response to interleukin-1 and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071456 and cellular response to hypoxia and biological process, IRAK and pelle, IRAK1 and IDBG-632573 and ENSBTAG00000016085 and 533953, IRAK1 and IDBG-90782 and ENSG00000184216 and 3654, Irak1 and IDBG-154747 and ENSMUSG00000031392 and 16179, leucine rich repeat containing receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0045087 and innate immune response and biological process this GO :0045323 and interleukin-1 receptor complex and cellular component this GO :0045893 and positive regulation of transcription, nuclei, this GO :0000187 and activation of MAPK activity and biological process this GO :0001959 and regulation of cytokine-mediated signaling pathway and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004704 and NF-kappaB-inducing kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005149 and interleukin-1 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005811 and lipid particle and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007178 and transmembrane receptor protein serine/threonine kinase signaling pathway and biological process this GO :0007250 and activation of NF-kappaB-inducing kinase activity and biological process this GO :0007254 and JNK cascade and biological process this GO :0007568 and aging and biological process this GO :0008152 and metabolic process and biological process this GO :0010008 and endosome membrane and cellular component this GO :0016020 and membrane and cellular component this GO :0016301 and kinase activity and molecular function this GO :0016567 and protein ubiquitination and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004704 : NF-kappaB-inducing kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005149 : interleukin-1 receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016301 : kinase activity and also this GO :0016772 : transferase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004704 : NF-kappaB-inducing kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0005149 : interleukin-1 receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016301 : kinase activity, this GO :0016772 : transferase activity, this GO :0031072 : heat shock protein binding, this GO :0042803 : protein homodimerization activity, this GO :0046982 : protein heterodimerization activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0031072 : heat shock protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0046982 : protein heterodimerization activity, transferring phosphorus-containing groups and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0031072 and heat shock protein binding and molecular function this GO :0031663 and lipopolysaccharide-mediated signaling pathway and biological process this GO :0032088 and negative regulation of NF-kappaB transcription factor activity and biological process this GO :0032494 and response to peptidoglycan and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034162 and toll-like receptor 9 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0034605 and cellular response to heat and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0035872 and nucleotide-binding domain, interleukin-1 receptor-associated kinase 1 |
| Identity: |
6112 |
| Gene: |
IRAK1 |
More about : IRAK1 |
| Long gene name: |
interleukin 1 receptor associated kinase 1 |
| Locus: |
Xq28 |
| Discovery year: |
1998-06-22 |
| GenBank acession: |
L76191 |
| Entrez gene record: |
3654 |
| Pubmed identfication: |
9374458 8599092 |
| Havana BLAST/BLAT: |
OTTHUMG00000024228 |
| Locus Specific Databases: |
Mental Retardation database |