| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Histone H2A, X Ser140 |
| Specificity: |
Histone H2A, This phosphorylation site is homologous to that of Ser140 in Mouse and Rat, X (Ser140) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser140 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Histone H2AX around the phosphorylation site of Ser140 |
| Gene ID: |
3014 |
| Swiss Prot: |
P16104 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
DNA accessibility is regulated via a complex set of post-translational modifications of histones, DNA repair, DNA replication and chromosomal stability, Histones thereby play a central role in transcription regulation, Nucleosomes wrap and compact DNA into chromatin, Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation, also called histone code, and nucleosome remodeling, limiting DNA accessibility to the cellular machineries which require DNA as a template, Variant histone H2A which replaces conventional H2A in a subset of nucleosomes |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
H2A, H2A/X, H2AFX, Histone H2A, Histone H2AX, H2AX, X, X |
| Also known as: |
Histone H2A, X (Ser140) Antibody |
| Other name: |
Anti-Histone H2A, X (Ser140) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
Histone H2A, X (Ser140) |
| Short name: |
Anti-Histone H2A, X (Ser140) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, X (Ser140), antibody to-Histone H2A |