| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
FHIT Tyr114 |
| Specificity: |
This antibody reacts specifically with FHIT (Tyr114) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr114 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Mitochondrion, Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human FHIT around the phosphorylation site of Tyr114 |
| Gene ID: |
2272 |
| Swiss Prot: |
P49789 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), Functions as tumor suppressor, Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis, Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways, but does not require its catalytic activity, but has extremely low activity with ATP, enhancing mitochondrial calcium uptake, it may in part come from the mitochondrial form, such as CCND1 and BIRC5, which sensitizes the low-affinity Ca(2+) transporters, Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FRA3B AP3Aase |
| Also known as: |
FHIT (Tyr114) Polyclonal Antibody |
| Other name: |
Anti-FHIT (Tyr114) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
FHIT (Tyr114) |
| Short name: |
Anti-FHIT (Tyr114) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-FHIT (Tyr114) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3701 |
| Gene: |
FHIT |
More about : FHIT |
| Long gene name: |
fragile histidine triad |
| Synonyms gene name: |
fragile histidine triad gene |
| Locus: |
3p14, 2 |
| Discovery year: |
1996-10-18 |
| GenBank acession: |
BC032336 |
| Entrez gene record: |
2272 |
| Pubmed identfication: |
8598045 9671749 26181368 |
| RefSeq identity: |
NM_002012 |
| Havana BLAST/BLAT: |
OTTHUMG00000158591 |