| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Dab1 Tyr220 |
| Specificity: |
This antibody reacts specifically with Dab1 (Tyr220) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr220 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Dab1 around the phosphorylation site of Tyr220 |
| Gene ID: |
1600 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus, Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin, Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, Phenotypically, Reelin-induced signaling during neural cortex development, Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1, Tau, Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases, and severe cerebellar hypoplasia, disruption of neuronal migration, likely indirectly, the mutation of this gene produces motor defects and ataxia, Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Disabled homolog 1, Disabled homolog 1 Drosophila, Scm, Scr, Scrambler, Yot, Yotari, Dab 1 |
| Also known as: |
Dab1 (Tyr220) Polyclonal Antibody |
| Other name: |
Anti-Dab1 (Tyr220) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Dab1 (Tyr220) |
| Short name: |
Anti-Dab1 (Tyr220) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-disabled homolog 1 (Drosophila) (Tyr220) (polyclonal) |
| Alternative technique: |
polyclonals |