| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BAD Ser75 |
| Specificity: |
This antibody reacts specifically with BAD (Ser75) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser75 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser75 |
| Gene ID: |
572 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Alternative splicing of this gene results in two transcript variants which encode the same isoform, Bad is found on the outer mitochondrial membrane and, Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins, JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis, The latter interaction is critical for Bad phosphorylation at serine 155, The phosphorylation status of Bad represents a key checkpoint for death or cell survival, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival, once phosphorylated in response to growth stimuli, thus preventing them from binding with BAX, translocates to the cytoplasm, Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
BCL2L8 BBC2 |
| Also known as: |
BAD (Ser75) Polyclonal Antibody |
| Other name: |
Anti-BAD (Ser75) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BAD (Ser75) |
| Short name: |
Anti-BAD (Ser75) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-BCL2-associated a this GO nist on cell death (Ser75) (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BAD and IDBG-53423 and ENSG00000002330 and 572, BBC2 and BCL2L8, BT, Bad and IDBG-137335 and ENSMUSG00000024959 and 12015, Cytoplasm, protein heterodimerization activity, this GO :0001666 and response to hypoxia and biological process this GO :0001836 and release of cytochrome c from mitochondria and biological process this GO :0005515 and protein binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005741 and mitochondrial outer membrane and cellular component this GO :0005829 and cytosol and cellular component this GO :0006007 and glucose catabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0008283 and cell proliferation and biological process this GO :0008289 and lipid binding and molecular function this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008625 and extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0008656 and cysteine-type endopeptidase activator activity involved in apoptotic process and molecular function this GO :0009725 and response to hormone and biological process this GO :0009749 and response to glucose and biological process this GO :0010033 and response to organic substance and biological process this GO :0010508 and positive regulation of autophagy and biological process this GO :0010918 and positive regulation of mitochondrial membrane potential and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0019050 and suppression by virus of host apoptotic process and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0019903 and protein phosphatase binding and molecular function this GO :0030346 and protein phosphatase 2B binding and molecular function this GO :0032024 and positive regulation of insulin secretion and biological process this GO :0032355 and response to estradiol and biological process this GO :0032570 and response to progesterone and biological process this GO :0033133 and positive regulation of glucokinase activity and biological process this GO :0033574 and response to testosterone and biological process this GO :0034201 and response to oleic acid and biological process this GO :0035774 and positive regulation of insulin secretion involved in cellular response to glucose stimulus and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042493 and response to drug and biological process this GO :0042542 and response to hydrogen peroxide and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0042981 and regulation of apoptotic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043200 and response to amino acid and biological process this GO :0043280 and positive regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043281 and regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043422 and protein kinase B binding and molecular function this GO :0044342 and type B pancreatic cell proliferation and biological process this GO :0045087 and innate immune response and biological process this GO :0045471 and response to ethanol and biological process this GO :0045579 and positive regulation of B cell differentiation and biological process this GO :0045582 and positive regulation of T cell differentiation and biological process this GO :0045862 and positive regulation of proteolysis and biological process this GO :0046031 and ADP metabolic process and biological process this GO :0046034 and ATP metabolic process and biological process this GO :0046902 and regulation of mitochondrial membrane permeability and biological process this GO :0046931 and pore complex assembly and biological process this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051592 and response to calcium ion and biological process this GO :0060139 and positive regulation of apoptotic process by virus and biological process this GO :0060154 and cellular process regulating host cell cycle in response to virus and biological process this GO :0071247 and cellular response to chromate and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0071316 and cellular response to nicotine and biological process this GO :0071396 and cellular response to lipid and biological process this GO :0071456 and cellular response to hypoxia and biological process this GO :0090200 and positive regulation of release of cytochrome c from mitochondria and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :0097202 and activation of cysteine-type endopeptidase activity and biological process this GO :1900740 and positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway and biological process this GO :1901216 and positive regulation of neuron death and biological process this GO :2000078 and positive regulation of type B pancreatic cell development and biological process this GO :2001244 and positive regulation of intrinsic apoptotic signaling pathway and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0005543 : phospholipid binding and also this GO :0008289 : lipid binding and also this GO :0008656 : cysteine-type endopeptidase activator activity involved in apoptotic process and also this GO :0019901 : protein kinase binding and also this GO :0019903 : protein phosphatase binding and also this GO :0030346 : protein phosphatase 2B binding and also this GO :0043422 : protein kinase B binding and also this GO :0046982 : protein heterodimerization activity, this GO :0005543 : phospholipid binding, this GO :0008289 : lipid binding, this GO :0008656 : cysteine-type endopeptidase activator activity involved in apoptotic process, this GO :0019901 : protein kinase binding, this GO :0019903 : protein phosphatase binding, this GO :0030346 : protein phosphatase 2B binding, this GO :0043422 : protein kinase B binding, this GO :0046982 : protein heterodimerization activity, 55802 and IDBG-643918 and ENSBTAG00000012511 and 615013, BCL2-associated a this GO nist of cell death |
| Identity: |
936 |
| Gene: |
BAD |
More about : BAD |
| Long gene name: |
BCL2 associated agonist of cell death |
| Locus: |
11q13, 1 |
| Discovery year: |
1997-10-16 |
| GenBank acession: |
AF021792 |
| Entrez gene record: |
572 |
| Pubmed identfication: |
8929532 |
| RefSeq identity: |
NM_032989 |
| Classification: |
BCL2 homology region 3 (BH3) only |
| Havana BLAST/BLAT: |
OTTHUMG00000134302 |