| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
P105 RB |
| Specificity: |
This antibody reacts specifically with P105 RB |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Rb/P105 RB |
| Gene ID: |
5925 |
| Swiss Prot: |
P06400 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a transcription repressor of E2F1 target genes, Controls histone H4 'Lys-2' trimethylation, Directly involved in heterochromatin formation by maintaining overall chromatin structure and, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity, In case of viral infections, In resting neurons, Inhibits the intrinsic kinase activity of TAF1, Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter, Promotes G-G1 transition when phosphorylated by CDK3/cyclin-C, RB1 is dephosphorylated by calcineurin, Recruits and targets histone methyltransferases SUV39H1, SUV42H1 and SUV42H2, The underphosphorylated, Upon calcium influx, active form of RB1 interacts with E2F1 and represses its transcription activity, in particular, interactions with SV4 large T antigen, leading to cell cycle arrest, leading to epigenetic transcriptional repression, that of constitutive heterochromatin by stabilizing histone methylation, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex, which leads to release of the repressor complex (By similarity), Key regulator of entry into cell division that acts as a tumor suppressor |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
LYT-10 p52 p105 NF-kB2 p49/p100 |
| Also known as: |
P105 RB Polyclonal Antibody |
| Other name: |
Anti-P105 RB Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
P105 RB |
| Short name: |
Anti-P105 RB (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-P105 RB (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7795 |
| Gene: |
NFKB2 |
More about : NFKB2 |
| Long gene name: |
nuclear factor kappa B subunit 2 |
| Synonyms gene name: |
nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) |
| Locus: |
10q24, 32 |
| Discovery year: |
1991-11-14 |
| GenBank acession: |
X61498 |
| Entrez gene record: |
4791 |
| Pubmed identfication: |
1876189 |
| Classification: |
Ankyrin repeat domain containing NF-kappa B complex subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000018962 |