| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BTK |
| Specificity: |
This antibody reacts specifically with BTK |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BTK |
| Gene ID: |
695 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3, BTK plays an important role in B cell development, Like other BTK family members, Src homology SH3 and SH2 domains, The PKC/BTK inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal BTK activity, The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223, Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, it contains a pleckstrin homology (PH) domain, leading to autophosphorylation at Tyr223 within the SH3 domain, transphosphorylation and subsequent activation, which is necessary for full activation, which results in reduced membrane recruitment, 4, 5-trisphosphate, Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ATK XLA PSCTK1 |
| Also known as: |
BTK Polyclonal Antibody |
| Other name: |
Anti-BTK Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BTK |
| Short name: |
Anti-BTK (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Bruton agammaglobulinemia tyrosine kinase (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
AGMX1 and AT and ATK and BPK and IMD1 and PSCTK1 and XLA, BTK and IDBG-633447 and ENSBTAG00000019250 and 533459, BTK and IDBG-79651 and ENSG00000010671 and 695, Btk and IDBG-171427 and ENSMUSG00000031264 and 12229, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0007249 and I-kappaB kinase/NF-kappaB signaling and biological process this GO :0007498 and mesoderm development and biological process this GO :0010033 and response to organic substance and biological process this GO :0016772 and transferase activity, nuclei, phosphatidylinositol-3, this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002250 and adaptive immune response and biological process this GO :0002553 and histamine secretion by mast cell and biological process this GO :0002721 and regulation of B cell cytokine production and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002902 and regulation of B cell apoptotic process and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005547 and phosphatidylinositol-3, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004715 : non-membrane spanning protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0005547 : phosphatidylinositol-3, this GO :0004713 : protein tyrosine kinase activity, this GO :0004715 : non-membrane spanning protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0005547 : phosphatidylinositol-3, this GO :0016772 : transferase activity, this GO :0042802 : identical protein binding, this GO :0046872 : metal ion binding, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0042802 : identical protein binding and also this GO :0046872 : metal ion binding, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019722 and calcium-mediated signaling and biological process this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034614 and cellular response to reactive oxygen species and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0042113 and B cell activation and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042802 and identical protein binding and molecular function this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045579 and positive regulation of B cell differentiation and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048469 and cell maturation and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050853 and B cell receptor signaling pathway and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0071226 and cellular response to molecule of fungal origin and biological process this GO :0097190 and apoptotic signaling pathway and biological process, 4, 4, 4, 5-trisphosphate binding, 5-trisphosphate binding and also this GO :0016772 : transferase activity, 5-trisphosphate binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, Bruton agammaglobulinemia tyrosine kinase |
| Identity: |
1133 |
| Gene: |
BTK |
More about : BTK |
| Long gene name: |
Bruton tyrosine kinase |
| Synonyms gene: |
AGMX1 IMD1 |
| Synonyms gene name: |
Bruton agammaglobulinemia tyrosine kinase |
| Synonyms name: |
Bruton', s tyrosine kinase |
| Locus: |
Xq22, 1 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
AK057105 |
| Entrez gene record: |
695 |
| Pubmed identfication: |
8380905 |
| RefSeq identity: |
NM_000061 |
| Classification: |
Pleckstrin homology domain containing SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000022022 |
| Locus Specific Databases: |
BTK base: Mutation registry for X-linked agammaglobulinemia Mental Retardation database LRG_128 |