| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPNMB |
| Specificity: |
This antibody reacts specifically with GPNMB |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GPNMB |
| Gene ID: |
10457 |
| Swiss Prot: |
Q14956 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
GPNMB may be involved in growth delay and reduction of metastatic potential, GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines, Two transcript variants encoding different isoforms have been found for this gene, [provided by RefSeq], a melanocyte-specific protein, The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NMB HGFIN |
| Also known as: |
GPNMB Polyclonal Antibody |
| Other name: |
Anti-GPNMB Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPNMB |
| Short name: |
Anti-GPNMB (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-glycoprotein (transmembrane) nmb (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, GPNMB and IDBG-9627 and ENSG00000136235 and 10457, Gpnmb and IDBG-146080 and ENSMUSG00000029816 and 93695, HGFIN and NMB, Plasma membranes, heparin binding, this GO :0001649 and osteoblast differentiation and biological process this GO :0005178 and integrin binding and molecular function this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0008201 and heparin binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0030282 and bone mineralization and biological process this GO :0042470 and melanosome and cellular component, this GO :0005178 : integrin binding, this GO :0005178 : integrin binding and also this GO :0008201 : heparin binding, this GO :0008201 : heparin binding, 99652 and IDBG-630320 and ENSBTAG00000000604 and 509600, glycoprotein (transmembrane) nmb |
| Identity: |
4462 |
| Gene: |
GPNMB |
More about : GPNMB |
| Long gene name: |
glycoprotein nmb |
| Synonyms gene name: |
glycoprotein (transmembrane) nmb |
| Synonyms name: |
transmembrane glycoprotein glycoprotein NMB glycoprotein nmb-like protein osteoactivin hematopoietic growth factor inducible neurokinin-1 glycoprotein nonmetastatic melanoma protein B |
| Locus: |
7p15, 3 |
| Discovery year: |
1999-10-26 |
| GenBank acession: |
X76534 |
| Entrez gene record: |
10457 |
| Pubmed identfication: |
7814155 |
| RefSeq identity: |
NM_001005340 |
| Havana BLAST/BLAT: |
OTTHUMG00000022811 |