| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
IRAK4 |
| Specificity: |
This antibody reacts specifically with IRAK4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human IRAK4 |
| Gene ID: |
51135 |
| Swiss Prot: |
Q9NWZ3 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Alternatively, In turn, Involved in Toll-like receptor (TLR) and IL-1R signaling pathways, Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1, Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections, Phosphorylates initially IRAK1, Then, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1, Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NY-REN-64 |
| Also known as: |
IRAK4 Polyclonal Antibody |
| Other name: |
Anti-IRAK4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IRAK4 |
| Short name: |
Anti-IRAK4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-interleukin-1 receptor-associated kinase 4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Extracellular, IRAK4 and IDBG-28022 and ENSG00000198001 and 51135, IRAK4 and IDBG-633310 and ENSBTAG00000021105 and 533692, Irak4 and IDBG-177236 and ENSMUSG00000059883 and 266632, this GO :0000287 and magnesium ion binding and molecular function this GO :0001816 and cytokine production and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005149 and interleukin-1 receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007254 and JNK cascade and biological process this GO :0010008 and endosome membrane and cellular component this GO :0016772 and transferase activity, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0004672 : protein kinase activity and also this GO :0004674 : protein serine/threonine kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0005149 : interleukin-1 receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004672 : protein kinase activity, this GO :0004674 : protein serine/threonine kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0005149 : interleukin-1 receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034146 and toll-like receptor 5 signaling pathway and biological process this GO :0034162 and toll-like receptor 9 signaling pathway and biological process this GO :0034166 and toll-like receptor 10 signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0045087 and innate immune response and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process, interleukin-1 receptor-associated kinase 4 |
| Identity: |
17967 |
| Gene: |
IRAK4 |
More about : IRAK4 |
| Long gene name: |
interleukin 1 receptor associated kinase 4 |
| Locus: |
12q12 |
| Discovery year: |
2002-09-27 |
| GenBank acession: |
AF155118 |
| Entrez gene record: |
51135 |
| Pubmed identfication: |
3772297 10508479 |
| Havana BLAST/BLAT: |
OTTHUMG00000169425 |
| Locus Specific Databases: |
IRAK4base: Mutation registry for IRAK4 deficiency LRG_75 |