| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Smad2 Ser465 |
| Specificity: |
This antibody reacts specifically with Smad2 (Ser465) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser465 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Smad2 around Ser465 |
| Gene ID: |
4087 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It belongs to the SMAD family of proteins, It interacts with SMAD anchor for receptor activation (SARA), It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor, Like many other TGF-Beta family members SMAD2 is involved in cell signalling, Mothers against DPP homolog 2, SMAD 2, SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase, SMAD2 modulates signals of activin and TGF-Beta's, Smad2 (Mothers against decapentaplegic homolog 2, The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4, as its name describes, is a homolog of the Drosophila gene: ", which belong to the TGF-Beta superfamily of modulators, ), Mothers against decepentaplegic", SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
JV18, JV18 1, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, SMAD, SMAD 2, SMAD 2, SMAD2, SMAD2_HUMAN, Smad2, Smad2S465, hMAD 2, hSMAD2, mothers against DPP homolog 2, p-Smad2Ser465, Smad2Ser465 |
| Also known as: |
Smad2 (Ser465) Polyclonal Antibody |
| Other name: |
Anti-Smad2 (Ser465) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Smad2 (Ser465) |
| Short name: |
Anti-Smad2 (Ser465) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SMAD family member 2 (Ser465) (polyclonal) |
| Alternative technique: |
polyclonals |