| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Ki-67 proliferation Marker |
| Specificity: |
This antibody reacts specifically with Ki-67 (Proliferation Marker) |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Ki67 |
| Gene ID: |
4288 |
| Swiss Prot: |
P46013 |
| Applications: |
IF(ICC), IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
G2 and M phase), In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index, It is absent in resting (G0) cells, Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index), Ki67 is routinely used as a neuronal marker of cell cycling and proliferation, S, The correlation between low Ki67 index and histologically low grade tumours is strong, expressed by proliferating cells in all phases of the active cell cycle (G1, Proliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Antigen KI-67, MIB-, MIB-1, MKI67, PPP1R15, KIA |
| Also known as: |
Ki-67 (Proliferation Marker) Antibody |
| Other name: |
Anti-Ki-67 (Proliferation Marker) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
Ki-67 (Proliferation |
| Short name: |
Anti-Ki-67 (Proliferation Marker) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Ki-67 (expantion detection labelled) |