| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
LIS1 |
| Specificity: |
This antibody reacts specifically with LIS1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human LIS1 |
| Gene ID: |
5048 |
| Swiss Prot: |
P43034 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules, May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing, May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end, Neuronal migration involves a process called nucleokinesis, Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity), Positively regulates the activity of the minus-end directed microtubule motor protein dynein, Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate, Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates, Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
LIS1 PAFAH NudF |
| Also known as: |
LIS1 Polyclonal Antibody |
| Other name: |
Anti-LIS1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
LIS1 |
| Short name: |
Anti-LIS1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-LIS1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
8574 |
| Gene: |
PAFAH1B1 |
More about : PAFAH1B1 |
| Long gene name: |
platelet activating factor acetylhydrolase 1b regulatory subunit 1 |
| Synonyms gene: |
MDCR MDS |
| Synonyms gene name: |
alpha subunit (45kD) Miller-Dieker syndrome chromosome region platelet-activating factor acetylhydrolase, alpha subunit 45kDa platelet-activating factor acetylhydrolase, isoform Ib, isoform Ib, isoform Ib, platelet-activating factor acetylhydrolase, regulatory subunit 1 (45kDa) , subunit 1 (45kDa) platelet-activating factor acetylhydrolase 1b |
| Synonyms name: |
lissencephaly-1 |
| Locus: |
17p13, 3 |
| Discovery year: |
1998-04-03 |
| GenBank acession: |
L25107 |
| Entrez gene record: |
5048 |
| Pubmed identfication: |
8355785 9063735 |
| RefSeq identity: |
NM_000430 |
| Classification: |
WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000177574 |