| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ZW10 peptide |
| Specificity: |
This antibody reacts specifically with ZW10 peptide |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from mouse ZW10 |
| Gene ID: |
9183 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Depletion from human cells demonstrates that the ZW10 complex is essential for stable binding of a Mad1-Mad2 complex to unattached kinetochores, In mitotic human cells ZW10 resides in a complex with Rod and Zwilch, It is part of the MIS12 complex, The encoded protein binds to centromeres during the prophase, This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division, Thus, ZW10 functions as a linker between the core structural elements of the outer kinetochore and components that catalyze generation of the mitotic checkpoint-derived ", Zwint-1, Zwint-1 is critical for recruiting ZW10 to unattached kinetochores, and early anaphase cell division stages and to kinetochore microtubules during metaphase, inhibitor, is part of a separate complex of structural kinetochore components including Mis12 and Ndc80-Hec1, metaphase, whereas another ZW10 partner, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis, The mitotic checkpoint ensures that chromosomes are divided equally between daughter cells and is a primary mechanism preventing the chromosome instability often seen in aneuploid human tumors, stop anaphase" |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KNTC1AP |
| Also known as: |
ZW10 peptide Polyclonal Antibody |
| Other name: |
Anti-ZW10 peptide Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Description: |
, The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs, The shortest peptides are dipeptides, These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), and unbranched synthetic peptide chains, consisting of 2 amino acids joined by a single peptide bond, continuous, followed by tripeptides, or in continue produced for custom peptide synthesis projects, tetra peptides, till polypeptides that are long, Peptides short amino acid chains or epitopes or blocking antagonists |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
ZW10 peptide |
| Short name: |
Anti-ZW10 peptide (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , peptides, whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ZW10 short protein sequence (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
13194 |
| Gene: |
ZW10 |
More about : ZW10 |
| Long gene name: |
zw10 kinetochore protein |
| Synonyms gene name: |
ZW10 (Drosophila) homolog, centromere/kinetochore protein ZW10, homolog (Drosophila) , kinetochore associated |
| Locus: |
11q23, 2 |
| Discovery year: |
1999-05-11 |
| GenBank acession: |
U54996 |
| Entrez gene record: |
9183 |
| Pubmed identfication: |
9298984 |
| RefSeq identity: |
NM_004724 |
| Havana BLAST/BLAT: |
OTTHUMG00000168190 |