| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
lamin A/C |
| Specificity: |
This antibody reacts specifically with lamin A/C |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human lamin A/C |
| Gene ID: |
4000 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A-type lamins and B-type lamins, Besides interactions with the nuclear membrane and other intermediate filaments, Eukaryotic chromatin is organised into loops, Lamins, Many biochemical and molecular features of lamins have been studied, Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane, One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus, The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, The nuclear lamins comprise a unique subclass of the intermediate filament protein family, They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, This organisation is thought to contribute to compaction of the chromatin and regulation of gene expression, Two main subtypes of nuclear lamins can be distinguished, a central rod of a-helices and a carboxyterminal globular domain, as part of the nuclear matrix, but their functions remain still largely undetermined, i, i, i, lamin A, lamin B1 and lamin B2, lamin C and lamin Adel10, lamins interact with the nuclear chromatin, may be involved in these processes since chromatin binding sites have been detected in both A- and B-type lamins, which are attached to the nuclear matrix, while the B-type lamins include two proteins arising from two distinct genes, Nuclear Envelope Marker An important part of the cell nucleus is formed by nuclear lamina, e, e, e |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HGPS MADA LMNA |
| Also known as: |
lamin A/C Polyclonal Antibody |
| Other name: |
Anti-lamin A/C Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
lamin A/C |
| Short name: |
Anti-lamin A/C (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-lamin A/C (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9450 |
| Gene: |
PRO1 |
More about : PRO1 |
| Long gene name: |
lamin A/C |
| Synonyms gene: |
LMN1 CMD1A LGMD1B LMNL1 |
| Synonyms gene name: |
cardiomyopathy, dilated 1A (autosomal dominant) limb girdle muscular dystrophy 1B (autosomal dominant) progeria 1 (Hutchinson-Gilford type) lamin A/C-like 1 |
| Synonyms name: |
mandibuloacral dysplasia type A |
| Locus: |
1q22 |
| Discovery year: |
1992-04-09 |
| GenBank acession: |
BC014507 |
| Entrez gene record: |
4000 |
| Pubmed identfication: |
8511676 8838815 12702809 |
| RefSeq identity: |
NM_170707 |
| Classification: |
Lamins |
| Havana BLAST/BLAT: |
OTTHUMG00000013961 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database Leiden Muscular Dystrophy Pages UMD Locus Specific Databases Leiden Muscular Dystrophy pages LRG_254 |