| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
EPH receptor A4 |
| Specificity: |
This antibody reacts specifically with EPH receptor A4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human EphA4 |
| Gene ID: |
2043 |
| Swiss Prot: |
P54764 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis, Additionally, Highly promiscuous, In addition to its role in axonal guidance plays a role in synaptic plasticity, In the nervous system, May also control the segregation of motor and sensory axons during neuromuscular circuit development, Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections, Rap and Rho GTPases activity, The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling, Upon activation by ephrin ligands, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium, leading to contact-dependent bidirectional signaling into neighboring cells, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation, Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
EPH |
| Also known as: |
EPH receptor A4 Polyclonal Antibody |
| Other name: |
Anti-EPH receptor A4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Description: |
2 or 3 and , However, In this sense, When such chemical-signals couple or bind to a receptor, a change in the electrical-activity of a cell, acetylcholine, am olfactory receptor is a protein-molecule that recognizes and responds to , an acetylcholine-receptor recognizes and responds to its endogenous-ligand, chemokinesor cytokines e, e, sometimes in pharmacology, such as enzymes, the term is also used to include other proteins that are drug-targets, they cause some form of cellular/tissue-response, transporters and ion-channels, The receptors are ligand binding factors of type 1, endogenous-chemical signals, g, g, protein-molecules , that receive chemical-signals from outside a cell |
| Gene target: |
EPH receptor A4 |
| Short name: |
Anti-EPH receptor A4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-EPH receptor A4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3385 |
| Gene: |
EPHA1 |
More about : EPHA1 |
| Long gene name: |
EPH receptor A1 |
| Synonyms gene: |
EPHT EPHT1 |
| Synonyms gene name: |
EphA1 |
| Locus: |
7q34-q35 |
| Discovery year: |
1989-05-16 |
| GenBank acession: |
M18391 |
| Entrez gene record: |
2041 |
| Pubmed identfication: |
9267020 |
| Classification: |
Sterile alpha motif domain containing Fibronectin type III domain containing EPH receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000155894 |