| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
E2F3 |
| Specificity: |
This antibody reacts specifically with E2F3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human E2F3 |
| Gene ID: |
1871 |
| Swiss Prot: |
O00716 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication, E2F3 binds specifically to RB1 in a cell-cycle dependent manner, Inhibits adipogenesis, The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase, probably through the repression of CEBPA binding to its target gene promoters (By similarity), Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
E2F3 Polyclonal Antibody |
| Other name: |
Anti-E2F3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
E2F3 |
| Short name: |
Anti-E2F3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-E2F3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3115 |
| Gene: |
E2F3 |
More about : E2F3 |
| Long gene name: |
E2F transcription factor 3 |
| Locus: |
6p22, 3 |
| Discovery year: |
1994-08-31 |
| GenBank acession: |
Y10479 |
| Entrez gene record: |
1871 |
| Pubmed identfication: |
8246996 |
| Classification: |
E2F transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000016389 |