| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
c-Raf/Raf1 |
| Specificity: |
This antibody reacts specifically with c-Raf/Raf1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane, Mitochondrion, Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human c Raf |
| Gene ID: |
5894 |
| Swiss Prot: |
P04049 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ADCY5 and ADCY6, Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death, Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity, Phosphorylates TNNT2/cardiac muscle troponin T, Phosphorylates adenylyl cyclases: ADCY2, Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2), Regulates Rho signaling and migration, Restricts caspase activation in response to selected stimuli, The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, and erythroid differentiation, and is required for normal wound healing, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, apoptosis, apoptosis (MAP3K5/ASK1 and STK3/MST2), by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75', differentiation, notably Fas stimulation, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, pathogen-mediated macrophage apoptosis, proliferation and angiogenesis (RB1), resulting in their activation, survival and oncogenic transformation, which induces down-regulation of OCLN, Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CMD1NN, CRAF, Proto-oncogene c-RAF, RAF, RAF proto-oncogene serine/threonine-protein kinase, RAF1, Raf-1, c-Raf, NS5 |
| Also known as: |
c-Raf/Raf1 Polyclonal Antibody |
| Other name: |
Anti-c-Raf/Raf1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
c-Raf/Raf1 |
| Short name: |
Anti-c-Raf/Raf1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-c-Raf/v-raf-1 murine leukemia viral oncogene homolog 1 (polyclonal) |
| Alternative technique: |
polyclonals |