| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
ERK1 Thr202/Tyr204+ERK2 Thr183/Tyr185 |
| Specificity: |
This antibody reacts specifically with ERK1(T202/Y204)+ERK2(T183/Y185) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr183/Tyr185 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185 |
| Gene ID: |
5594, 5595 |
| Swiss Prot: |
P28482 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
MAP kinases, The activation of this kinase requires its phosphorylation by upstream kinases, Two alternatively spliced transcript variants encoding the same protein, Upon activation, act as an integration point for multiple biochemical signals, also known as extracellular signal-regulated kinases (ERKs), and are involved in a wide variety of cellular processes such as proliferation, but differing in the UTRs, differentiation, have been reported for this gene, this kinase translocates to the nucleus of the stimulated cells, transcription regulation and development, where it phosphorylates nuclear targets, The protein encoded by this gene is a member of the MAP kinase family |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ERK ERK2 p41mapk MAPK2 |
| Also known as: |
ERK1(T202/Y204)+ERK2(T183/Y185) Antibody |
| Other name: |
Anti-ERK1(T202/Y204)+ERK2(T183/Y185) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
ERK1(T202/Y204)+ERK2(T183/Y185) |
| Short name: |
Anti-ERK1(T202/Y204)+ERK2(T183/Y185) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-ERK1(T202/Y204)+ERK2(T183/Y185) |
| Identity: |
6871 |
| Gene: |
MAPK1 |
More about : MAPK1 |
| Long gene name: |
mitogen-activated protein kinase 1 |
| Synonyms gene: |
PRKM2 PRKM1 |
| Locus: |
22q11, 22 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
M84489 |
| Entrez gene record: |
5594 |
| Classification: |
Mitogen-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000030508 |
| Locus Specific Databases: |
LRG_786 |