| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Patched/PTCH |
| Specificity: |
This antibody reacts specifically with Patched/PTCH |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Patched/PTCH |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome, During development PTCH is found in all major target tissues of sonic hedgehog, It is also expressed in tumor cells but not in normal skin, PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal, PTCH is expressed in the adult brain, and tissues surrounding the zone of polarizing activity of the limb bud, as inactivation of this protein is probably a necessary, heart, if not sufficient step for tumorigenesis, indian hedgehog (IHH) and desert hedgehog (DHH), liver, lung, pancreas and kidney, placenta, skeletal muscle, somites, such as the ventral neural tube, PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), PTCH has a tumor suppressor function |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
BCNS |
| Also known as: |
Patched/PTCH Polyclonal Antibody |
| Other name: |
Anti-Patched/PTCH Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Patched/PTCH |
| Short name: |
Anti-Patched/PTCH (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Patched/PTCH (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9585 |
| Gene: |
PTCH1 |
More about : PTCH1 |
| Long gene name: |
patched 1 |
| Synonyms gene: |
NBCCS PTCH |
| Synonyms gene name: |
patched (Drosophila) homolog patched homolog (Drosophila) patched homolog 1 (Drosophila) |
| Locus: |
9q22, 32 |
| Discovery year: |
1996-07-19 |
| GenBank acession: |
AI494442 |
| Entrez gene record: |
5727 |
| Pubmed identfication: |
8658145 |
| RefSeq identity: |
NM_000264 |
| Havana BLAST/BLAT: |
OTTHUMG00000020280 |
| Locus Specific Databases: |
LRG_515 |