| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
IDH3B |
| Specificity: |
This antibody reacts specifically with IDH3B |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human IDH3B |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Each isozyme is a heterotetramer that is composed of two alpha subunits, Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, Jul 2008], NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle, The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase, These enzymes belong to two distinct subclasses, Three alternatively spliced transcript variants encoding different isoforms have been described for this gene, [provided by RefSeq, and one gamma subunit, and two NADP(+)-dependent isocitrate dehydrogenases, one beta subunit, one of which is mitochondrial and the other predominantly cytosolic, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+), which localize to the mitochondrial matrix, Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RP46 |
| Also known as: |
IDH3B Polyclonal Antibody |
| Other name: |
Anti-IDH3B Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IDH3B |
| Short name: |
Anti-IDH3B (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-IDH3B (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
5385 |
| Gene: |
IDH3B |
More about : IDH3B |
| Long gene name: |
isocitrate dehydrogenase 3 (NAD(+)) beta |
| Synonyms gene name: |
isocitrate dehydrogenase 3 (NAD+) beta |
| Locus: |
20p13 |
| Discovery year: |
1995-11-02 |
| Entrez gene record: |
3420 |
| Pubmed identfication: |
10575215 |
| Havana BLAST/BLAT: |
OTTHUMG00000031699 |