| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
hHR23b |
| Specificity: |
This antibody reacts specifically with hHR23b |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human hHR23b |
| Gene ID: |
5887 |
| Swiss Prot: |
P54727 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binds to polyubiquitin chains, Cooperatively with CETN2 appears to stabilize XPC, Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation, In vitro, Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair, Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex, May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome, May protect XPC from proteasomal degradation, Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome, RPA and the TFIIH complex, The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, The orientation of XPC complex binding appears to be crucial for inducing a productive NER, XPA, XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery, is part of the pre-incision (or initial recognition) complex, it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts, mismatched bubbles or single-stranded overhangs, the XPC:RAD23B dimer is sufficient to initiate NER, Multiubiquitin chain receptor involved in modulation of proteasomal degradation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HHR23B P58 HR23B |
| Also known as: |
hHR23b Polyclonal Antibody |
| Other name: |
Anti-hHR23b Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
hHR23b |
| Short name: |
Anti-hHR23b (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-hHR23b (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9813 |
| Gene: |
RAD23B |
More about : RAD23B |
| Long gene name: |
RAD23 homolog B, nucleotide excision repair protein |
| Synonyms gene name: |
RAD23 (S, cerevisiae) , cerevisiae) homolog B RAD23 homolog B (S |
| Synonyms name: |
XP-C repair complementing protein XP-C repair complementing complex 58 kDa |
| Locus: |
9q31, 2 |
| Discovery year: |
1994-07-20 |
| Entrez gene record: |
5887 |
| Pubmed identfication: |
7851894 8168482 |
| RefSeq identity: |
NM_002874 |
| Classification: |
Nucleotide excision repair |
| Havana BLAST/BLAT: |
OTTHUMG00000020446 |