| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GPR161 |
| Specificity: |
This antibody reacts specifically with GPR161 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GPR161 |
| Gene ID: |
23432 |
| Swiss Prot: |
Q8N6U8 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In presence of SHH, Its ligand is unknown (By similarity), Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regulator of the PKA-dependent basal repression machinery in Shh signaling by increasing cAMP levels, it is removed from primary cilia and is internalized into recycling endosomes, leading to promote the PKA-dependent processing of GLI3 into GLI3R and repress the Shh signaling, preventing its activity and allowing activation of the Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development, Key negative regulator of Shh signaling |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RE2 |
| Also known as: |
GPR161 Polyclonal Antibody |
| Other name: |
Anti-GPR161 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GPR161 |
| Short name: |
Anti-GPR161 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GPR161 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
23694 |
| Gene: |
GPR161 |
More about : GPR161 |
| Long gene name: |
G protein-coupled receptor 161 |
| Locus: |
1q24, 2 |
| Discovery year: |
2003-12-01 |
| GenBank acession: |
AF091890 |
| Entrez gene record: |
23432 |
| Pubmed identfication: |
11959142 |
| RefSeq identity: |
NM_007369 |
| Classification: |
Class A orphans , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000034651 |