| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Zonula occludens protein 3 |
| Specificity: |
This antibody reacts specifically with Zonula occludens protein 3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Zonula occludens protein 3 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
In addition, Many of the tight junction-associated proteins are members of the membrane-associated guanylate kinase (MAGUK) family and include occludin, These proteins are thought to have both structural and signaling roles, ZO-1, ZO-1 expression is significantly reduced in many breast cancer lines (8), ZO-1 forms complexes with either ZO-2 or ZO-3 (4), ZO-2 and ZO-3 (1, ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, ZO-2 is not expressed in nonepithelial tissue (2, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, and unlike ZO-1, at tight junction stands, occludin and F-actin, the SH3 domain and the guanylate kinase (GuK) domain (3), these proteins can also associate with claudin, where they provide a linkage between the actin cytoskeleton and the tight junction (5-7), which is also expressed at cell junctions of cardiac myocytes, 2), 3), Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
TJP3, Tight junction protein ZO-3, Tight junction protein ZO3, ZO3, ZO3_HUMAN, Zona occludens protein 3, Zonula occludens protein 3, Tight junction protein 3, ZO-3 |
| Also known as: |
Zonula occludens protein 3 Antibody |
| Other name: |
Anti-Zonula occludens protein 3 |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
Zonula occludens protein 3 |
| Short name: |
Fluor 594, Anti-Zonula occludens protein 3 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Zonula occludens protein 3 |