| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NSP3 |
| Specificity: |
This antibody reacts specifically with NSP3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NSP3 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
FAN (for factor associated with neutral sphingomyelinase (N-SMase) activation) is an intermediate protein that interacts with TNF-R1 to initiate TNF signaling events, FAN binds to TNF-R1 at the cytoplasmic NSD (N-SMase activating domain), FAN contains four carboxy-terminal WD-repeat domains which appear to be involved in protein-protein interaction, N-SMase has been shown to be involved in TNF-induced Raf-1 activation (6), The FAN WD-repeats may mediate the interaction between FAN and TNF-R1 (5), The majority of TNF functions are primarily mediated through TNF-R1 (1, These receptors, are expressed on most cell types (1, designated TNF-R1 and TNF-R2, which results in the initiation of the N-SMase pathway (5), 2), 3), 4), Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors (1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NSP3 |
| Also known as: |
NSP3 Polyclonal Antibody |
| Other name: |
Anti-NSP3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NSP3 |
| Short name: |
Anti-NSP3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NSP3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
16884 |
| Gene: |
SH2D3C |
More about : SH2D3C |
| Long gene name: |
SH2 domain containing 3C |
| Synonyms gene name: |
SH2 domain-containing 3C |
| Locus: |
9q34, 11 |
| Discovery year: |
2001-11-19 |
| GenBank acession: |
AF124251 |
| Entrez gene record: |
10044 |
| Pubmed identfication: |
10187783 |
| RefSeq identity: |
NM_005489 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020717 |